GC-MS-based profiling and ameliorative potential of carissa opaca stapf ex haines fruit against cardiac and testicular toxicity: An In vivo study

Fruit of Carissa opaca Stapf ex Haines (C. opaca) is a feed additive and commonly used against cardiac dysfunction, fever, asthma, diarrhea, gastrointestinal ailments, skin diseases. In this study, we aimed to evaluate the metabolic profile antioxidant potential C. fruit carbon tetrachloride (CCl4)-induced cardiotoxicity testicular toxicity in rats. Gas Chromatoghraphy-Mass Spectrometry (GC-MS) analysis for identification profile, followed by methanolic extract its derived fractions including n-hexane, ethyl acetate, chloroform, butanol, aqueous were assess fruits. Ten groups rats received different treatments got evaluated enzymes, histological architecture, serum hormonal levels. GC-MS showed presence some bioactive metabolites like cyclodecane, diethyl 2,6-pyridine dicarboxylate, tetrahydro-geraniol, S-[2-[N, N-Dimethylamino]ethyl]morpoline, 2,3-Methylenedioxyphenol, alpha-d-Glucopyranoside, 5,10-Diethoxy-2,3,7,8-tetrahydro-1H, 6H-dipyrrolo [1,2-a; 1′,2′-d] pyrazine 1,3-Benzothiazol-2(3H)-one,3-(3,3-dimethyl-1-oxobutyl) that corresponds medicinal properties fruit. Prepared fruits mitigated induced CCl4 heart tissues Oxidative stress was caused inhibition activities glutathione other enzymes body, while on hand elevating levels nitrite hydrogen peroxide. Treatment with normalized reproductive hormones, free radicals thus restoring histopathological enzymatic biomarkers towards normal group. The study supports indigenous use as an alternative medicine dysfunction providing scientific evidence protection CCl4-induced injuries, it also concludes defensive role tissues.